DNA

Part:BBa_K2940007:Design

Designed by: Ping CHANG   Group: iGEM19_Edinburgh_OG   (2019-10-16)


Secreted dye-degrading peroxidase（DyP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 10
    Illegal NheI site found at 33
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 155
    Illegal BsaI site found at 164
    Illegal BsaI site found at 1130
    Illegal SapI site found at 560
    Illegal SapI site found at 918
    Illegal SapI.rc site found at 1074


Design Notes

ABTS assay to determine improved activity when compared to wild type.



Source

The coding sequence of this part was reference from NCBI (NC_000964.3).

References